Fig. 2. A. Schematic illustration of the sgRNA-target DNA complex - (N- any nucleotide DNA- crRNA targeting sequence, duplex crRNA:tracrRNA sequence, and tri-loop tracrRNA sequence). B. Cartoon-surface representations of three-dimensional structure of sgRNA ID 5Y36 from PDB database. Structural image was prepared with the PyMOL Molecular Graphics System, Version 2.0 Schrödinger, LLC and Adobe Illustrator.